Understanding single-molecule reactions using nanopore-based techniques

Nature Chemistry, Published online: 30 September 2025; doi:10.1038/s41557-025-01905-w

Real-time single-molecule observation provides insights into transient intermediates that are normally inaccessible using ensemble measurements. This Review discusses the use of nanopores as both single-molecule nanoreactors and single-molecule sensors for elucidating reaction pathways and kinetics, while highlighting their potential to control covalent reactions under confinement.

​Nature Chemistry, Published online: 30 September 2025; doi:10.1038/s41557-025-01905-wReal-time single-molecule observation provides insights into transient intermediates that are normally inaccessible using ensemble measurements. This Review discusses the use of nanopores as both single-molecule nanoreactors and single-molecule sensors for elucidating reaction pathways and kinetics, while highlighting their potential to control covalent reactions under confinement. Read More

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