Nature Chemistry, Published online: 02 October 2025; doi:10.1038/s41557-025-01954-1
Pseudokinases are non-canonical protein-kinase-like proteins deficient in kinase activity, few of which have enzymatic activity that differs from phosphorylation. Now a pseudokinase-enabled cyclization activity for the biosynthesis of ribosomally synthesized and post-translationally modified peptides has been observed. Here pseudokinases can catalyse a Michael addition for (ene)thioether crosslinking through a sandwich-like substrate-assisted process.
Nature Chemistry, Published online: 02 October 2025; doi:10.1038/s41557-025-01954-1Pseudokinases are non-canonical protein-kinase-like proteins deficient in kinase activity, few of which have enzymatic activity that differs from phosphorylation. Now a pseudokinase-enabled cyclization activity for the biosynthesis of ribosomally synthesized and post-translationally modified peptides has been observed. Here pseudokinases can catalyse a Michael addition for (ene)thioether crosslinking through a sandwich-like substrate-assisted process. Read More
