Improving somatic variant calling with long-read technologies and public datasets

Nature Biotechnology, Published online: 16 October 2025; doi:10.1038/s41587-025-02860-0

Challenges in somatic variant calling include a lack of long-read variant callers and of publicly available benchmarking datasets. We developed DeepSomatic, a somatic variant caller for short- and long-read technologies, and created seven somatic variant benchmarks derived from cancer cell lines, which we make available as a public database: CASTLE-panel.

​Nature Biotechnology, Published online: 16 October 2025; doi:10.1038/s41587-025-02860-0Challenges in somatic variant calling include a lack of long-read variant callers and of publicly available benchmarking datasets. We developed DeepSomatic, a somatic variant caller for short- and long-read technologies, and created seven somatic variant benchmarks derived from cancer cell lines, which we make available as a public database: CASTLE-panel. Read More

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